Product Summary
Size: 96T,48T
Sensitivity: 1.27pmol/L
Detection range: 2-180pmol/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product Summary
Size: 96T,48T
Sensitivity: 1.27pmol/L
Detection range: 2-180pmol/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Glucagon-Like Peptide 1
Code
E0022Hu
Assay type
Sandwich
Size
96T,48T
Sensitivity
1.27pmol/L
Detection range
2-180pmol/L
Sample type
Serum, plasma, cell culture supernates
Species
Human
Storage
2-8oC
Assay time
1h 30m
Background
[Glucagon]: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. [Glucagon-like Peptide 1]: Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed: 22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). [Glucagon-like Peptide 2]: Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. [Oxyntomodulin]: Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. [Glicentin]: May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
UniProt accession
MASS(Da)
20909
GeneID
2641
Synonyms
GCG;Glicentin-related polypeptide;GLP-1;GLP-1(7-36);GLP-1(7-37);GLP-2;Glucagon;Glucagon-like Peptide 1;Glucagon-like Peptide 1(7-36);Glucagon-like Peptide 1(7-37);Glucagon-like Peptide 2;GRPP;Incretin hormone;OXM;OXY;Oxyntomodulin;Pro-glucagon Glicentin
Gene names
GCG
Research area
Metabolism
Target protein
GCG
Components
Components Quantity Pre-coated ELISA Plate 12 wells * 8 detachable strips Standard solution 0.5ml x1 Standard diluent 3ml x1 Streptavidin-HRP 6ml x1 Stop solution 6ml x1 Substrate solution A 6ml x1 Substrate solution B 6ml x1 Wash buffer concentrate (25x) 20ml x1 Biotinylated antibody 1ml x1
Protocol data
These standard curves of E0022Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration O.D. Average Corrected 96pmol/L 1.796 1.793 1.748 1.79 48pmol/L 0.974 0.93 0.886 0.887 24pmol/L 0.466 0.543 0.499 0.621 12pmol/L 0.277 0.247 0.202 0.217 6pmol/L 0.073 0.066 0.022 0.06 0pmol/L 0.03 0.045 0 0.06
Precision
We measured random samples of E0022Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay Sample n Mean Standard Deviation CV% Intra-Assay 1 18 87.2 2.41 2.8 Intra-Assay 2 18 17.5 0.9 5.2 Intra-Assay 3 18 37.1 1.46 3.9
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